Journal: Scientific Data

Article Title: Taxonomy, function and plasmids of microbial soil communities of Polish salt graduation towers

doi: 10.1038/s41597-025-05989-4

Figure Lengend Snippet: Schematic representation of the study workflow. Soil samples from three Polish salt graduation towers underwent parallel processing: (I) direct DNA extraction for metagenomic and 16S rRNA sequencing; (II) enrichment cultures leading to WGS and hybrid plasmidome sequencing. (III) physicochemical analyses including atomic absorption spectroscopy (AAS) for Na, K, Ca, Fe concentrations, CHNS analysis for total carbon, hydrogen, and nitrogen, and sulfur, and electrical conductivity (EC) measurement for soluble salt levels.

Article Snippet: The sequencing was performed using PacBio Revio platform, with Kinnex 16S rRNA library preparation kit (Novogene, Cambridge, UK) and using the universal bacterial primers 27 F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492 R (5′-GNTACCTTGTTACGACTT-3′).

Techniques: DNA Extraction, Sequencing, Atomic Absorption Spectroscopy

Journal: Scientific Data

Article Title: Taxonomy, function and plasmids of microbial soil communities of Polish salt graduation towers

doi: 10.1038/s41597-025-05989-4

Figure Lengend Snippet: Relative abundance of bacterial classes based on 16S rRNA gene amplicon sequencing (ASVs) across the nine soil samples.

Article Snippet: The sequencing was performed using PacBio Revio platform, with Kinnex 16S rRNA library preparation kit (Novogene, Cambridge, UK) and using the universal bacterial primers 27 F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492 R (5′-GNTACCTTGTTACGACTT-3′).

Techniques: Amplification, Sequencing